Biofuel production is attracting attention due to the recent rise in crude oil prices and the reduction of CO2 emissions. We succeeded in producing alcohol that cannot be produced by wild-type E. coli. E. coli is suitable for alcohol production because of its fast growth rate and easy genetic recombination. In order to improve biofuel productivity, the metabolism of E. coli is calculated using computer simulation, and a synthesis circuit is designed. In addition, alcohol fuel causes E. coli growth inhibition, so it is necessary to breed alcohol resistant E. coli. Microarray and proteome data are analyzed with bioinformatics technology and used to design synthetic circuits.
